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GaussPy implements the Autonomous Gaussian Decomposition (AGD) algorithm, which uses computer vision and machine learning techniques to provide optimized initial guesses for the parameters of a multi-component Gaussian model automatically and efficiently. The speed and adaptability of AGD allow it to interpret large volumes of spectral data efficiently. Although it was initially designed for applications in radio astrophysics, AGD can be used to search for one-dimensional Gaussian (or any other single-peaked spectral profile)-shaped components in any data set. To determine how many Gaussian functions to include in a model and what their parameters are, AGD uses a technique called derivative spectroscopy. The derivatives of a spectrum can efficiently identify shapes within that spectrum corresponding to the underlying model, including gradients, curvature and edges.