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PyExoCross, a Python adaptation of ExoCross (ascl:1803.014), post-processes molecular line lists generated by ExoMol, HITRAN, and HITEMP and other similar initiatives. It generates absorption and emission spectra and other properties, including partition functions, specific heats, and cooling functions, based on molecular line lists. The code also calculates cross sections with four line profiles: Doppler, Gaussian, Lorentzian, and Voigt. PyExoCross can convert data format between ExoMol and HITRAN, and supports importing and exporting line lists in the ExoMol and HITRAN/HITEMP formats.