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GAMER (GPU-accelerated Adaptive MEsh Refinement) serves as a general-purpose adaptive mesh refinement + GPU framework and solves hydrodynamics with self-gravity. The code supports adaptive mesh refinement (AMR), hydrodynamics with self-gravity, and a variety of GPU-accelerated hydrodynamic and Poisson solvers. It also supports hybrid OpenMP/MPI/GPU parallelization, concurrent CPU/GPU execution for performance optimization, and Hilbert space-filling curve for load balance. Although the code is designed for simulating galaxy formation, it can be easily modified to solve a variety of applications with different governing equations. All optimization strategies implemented in the code can be inherited straightforwardly.